1 00:00:07,970 --> 00:00:05,740 [Music] 2 00:00:10,520 --> 00:00:07,980 thank you very much for the introduction 3 00:00:13,190 --> 00:00:10,530 and so what I'm presenting today 4 00:00:15,350 --> 00:00:13,200 actually isn't a research that we've 5 00:00:18,800 --> 00:00:15,360 done it's a hypothesis that we put forth 6 00:00:22,310 --> 00:00:18,810 while we were at a summer astrobiology 7 00:00:25,400 --> 00:00:22,320 school so what is astrobiology summer 8 00:00:27,859 --> 00:00:25,410 school so you might have received emails 9 00:00:30,410 --> 00:00:27,869 if you're on the NASA Astrobiology 10 00:00:32,330 --> 00:00:30,420 Institute newsletter about this 11 00:00:35,750 --> 00:00:32,340 basically it's held every year in 12 00:00:39,350 --> 00:00:35,760 Santander Spain and you get three days 13 00:00:41,720 --> 00:00:39,360 of astrobiology lectures and then a 14 00:00:44,060 --> 00:00:41,730 fourth day and we go out to an excursion 15 00:00:46,040 --> 00:00:44,070 to an astrobiology related site but we 16 00:00:48,770 --> 00:00:46,050 also have these projects that we work on 17 00:00:50,930 --> 00:00:48,780 throughout the week and then our project 18 00:00:51,889 --> 00:00:50,940 eventually kind of progressed to 19 00:00:53,810 --> 00:00:51,899 something a little bit more that we 20 00:00:55,840 --> 00:00:53,820 continued working on after the 21 00:00:59,029 --> 00:00:55,850 astrobiology summary school and then 22 00:01:01,479 --> 00:00:59,039 something cool about astrobiology summer 23 00:01:04,759 --> 00:01:01,489 schools outside of learning things about 24 00:01:07,789 --> 00:01:04,769 astrobiology you make awesome friends as 25 00:01:10,490 --> 00:01:07,799 shown here and these three guys helped 26 00:01:16,070 --> 00:01:10,500 me escape Spain I was a little bit stuck 27 00:01:21,910 --> 00:01:16,080 and a Peterloo Divya helped me catch a 28 00:01:25,780 --> 00:01:21,920 train back to the airport in Madrid but 29 00:01:29,120 --> 00:01:25,790 what was the basic idea here during our 30 00:01:31,760 --> 00:01:29,130 astrobiology summary school so it 31 00:01:35,600 --> 00:01:31,770 focused if you could simplify everything 32 00:01:38,920 --> 00:01:35,610 here we basically discussed what life 33 00:01:41,960 --> 00:01:38,930 neat what life actually needed and 34 00:01:44,749 --> 00:01:41,970 simple components so first thing is a 35 00:01:46,910 --> 00:01:44,759 liquid solvent so most people think of 36 00:01:50,120 --> 00:01:46,920 water but it doesn't necessarily have to 37 00:01:52,580 --> 00:01:50,130 be water according to some people so a 38 00:01:55,600 --> 00:01:52,590 source of nutrients and then of course 39 00:01:57,859 --> 00:01:55,610 energy don't have energy iron 40 00:02:00,999 --> 00:01:57,869 thermodynamic equilibrium just like 41 00:02:04,240 --> 00:02:01,009 nessie here entered back in Memorial Day 42 00:02:11,270 --> 00:02:04,250 so it definitely is a source of energy 43 00:02:12,510 --> 00:02:11,280 to power life and then our group was the 44 00:02:15,530 --> 00:02:12,520 energy group and 45 00:02:20,580 --> 00:02:15,540 so we discussed about how life might 46 00:02:24,120 --> 00:02:20,590 utilize beta decay or a protocol 47 00:02:27,570 --> 00:02:24,130 radiation of the planet planetary 48 00:02:29,130 --> 00:02:27,580 magnetospheres and one of our advisors 49 00:02:30,690 --> 00:02:29,140 came up to us and she's like hey you 50 00:02:33,060 --> 00:02:30,700 guys should write a hypothesis paper 51 00:02:35,190 --> 00:02:33,070 about this and so what's a hypothesis 52 00:02:36,360 --> 00:02:35,200 paper well it's exactly what it sounds 53 00:02:38,640 --> 00:02:36,370 like and I'm just going to go through 54 00:02:40,070 --> 00:02:38,650 some examples of what other people have 55 00:02:45,120 --> 00:02:40,080 done so 56 00:02:49,350 --> 00:02:45,130 Raynald at all in 1983 their hypothesis 57 00:02:51,570 --> 00:02:49,360 was that life might utilize the planet 58 00:02:54,990 --> 00:02:51,580 planetary magnetospheres Jupiter as a 59 00:02:57,810 --> 00:02:55,000 source of life basically having the 60 00:03:01,140 --> 00:02:57,820 particle radiation hit the surface of 61 00:03:05,130 --> 00:03:01,150 the ice on one side and having that 62 00:03:08,430 --> 00:03:05,140 current go go down through subsurface 63 00:03:11,220 --> 00:03:08,440 ocean forming hydrogen and oxygen 64 00:03:13,590 --> 00:03:11,230 basically splitting water apart that 65 00:03:16,920 --> 00:03:13,600 that would then be able to power life 66 00:03:20,430 --> 00:03:16,930 and then Hitler and Phillips and 2001 67 00:03:23,280 --> 00:03:20,440 also using our particle radiation at 68 00:03:26,699 --> 00:03:23,290 this time talking about how organics 69 00:03:29,100 --> 00:03:26,709 might form on the surface of Europa and 70 00:03:30,920 --> 00:03:29,110 then that getting cycled into the 71 00:03:34,920 --> 00:03:30,930 subsurface ocean that can then be 72 00:03:38,910 --> 00:03:34,930 utilized by life more recently a John 73 00:03:40,949 --> 00:03:38,920 Johnson at all in 2014 discussed out of 74 00:03:45,680 --> 00:03:40,959 the workman Reynolds effect basically 75 00:03:50,210 --> 00:03:45,690 what that is is when ice freezes it 76 00:03:52,710 --> 00:03:50,220 freezes in some cations or anions 77 00:03:57,810 --> 00:03:52,720 preferentially depending on the 78 00:03:59,250 --> 00:03:57,820 conditions so you can form a voltage 79 00:04:01,440 --> 00:03:59,260 there that where you can also split 80 00:04:06,750 --> 00:04:01,450 water and form hydrogen and oxygen and 81 00:04:08,699 --> 00:04:06,760 then recently here actually in 2015 82 00:04:12,180 --> 00:04:08,709 discussed how you can use a particle 83 00:04:15,510 --> 00:04:12,190 radiation on Mars basically forming a 84 00:04:18,140 --> 00:04:15,520 secondary particles I can then go down 85 00:04:23,330 --> 00:04:18,150 to the surface 86 00:04:27,710 --> 00:04:23,340 and help power subsurface microbes on 87 00:04:31,909 --> 00:04:27,720 Mars so our hypothesis paper kind of 88 00:04:35,980 --> 00:04:31,919 adult with the idea of organisms using a 89 00:04:37,999 --> 00:04:35,990 current directly so this isn't a 90 00:04:39,950 --> 00:04:38,009 completely crazy concept because 91 00:04:43,790 --> 00:04:39,960 terrestrial organisms go ahead and do 92 00:04:47,120 --> 00:04:43,800 that so we have shelwyn ella and geo 93 00:04:49,969 --> 00:04:47,130 vector as being the first couple 94 00:04:53,600 --> 00:04:49,979 organisms that can use an electric 95 00:04:56,330 --> 00:04:53,610 current directly from a cathode and then 96 00:04:58,909 --> 00:04:56,340 basically take oxidized products to 97 00:05:01,629 --> 00:04:58,919 create reduce products and then somatic 98 00:05:07,460 --> 00:05:01,639 and Frank's here is a good review paper 99 00:05:09,200 --> 00:05:07,470 on that topic and so with this 100 00:05:13,580 --> 00:05:09,210 hypothesis we kind of need a head 101 00:05:19,370 --> 00:05:13,590 everything that we needed to basically 102 00:05:22,820 --> 00:05:19,380 propose organisms using current on icy 103 00:05:25,040 --> 00:05:22,830 world so we do have a liquid solvent and 104 00:05:28,730 --> 00:05:25,050 some of them in the case of europa you 105 00:05:33,800 --> 00:05:28,740 know a kilometers down or within the 106 00:05:36,290 --> 00:05:33,810 surface just because you know it's so 107 00:05:38,420 --> 00:05:36,300 cold at a 150 Kelvin on water is 108 00:05:40,100 --> 00:05:38,430 definitely frozen on the surface here 109 00:05:42,680 --> 00:05:40,110 but if you're not utilizing water as a 110 00:05:45,290 --> 00:05:42,690 solvent that becomes less of an issue 111 00:05:47,689 --> 00:05:45,300 definitely have your source of nutrients 112 00:05:49,460 --> 00:05:47,699 on a number of icy worlds and then the 113 00:05:52,670 --> 00:05:49,470 source of energy that we were using was 114 00:05:55,820 --> 00:05:52,680 particle radiation but there's an issue 115 00:06:00,980 --> 00:05:55,830 with particle radiation because most of 116 00:06:03,129 --> 00:06:00,990 it is so energetic enough that it you 117 00:06:07,760 --> 00:06:03,139 know as mentioned ionizes everything and 118 00:06:12,339 --> 00:06:07,770 destroys molecules that you're using so 119 00:06:15,830 --> 00:06:12,349 what we proposed was that energy can be 120 00:06:18,200 --> 00:06:15,840 dissipated here by having the electrons 121 00:06:20,240 --> 00:06:18,210 come in and forming secondary electrons 122 00:06:23,899 --> 00:06:20,250 which would be lower in energy then 123 00:06:25,670 --> 00:06:23,909 eventually going so low in the energy of 124 00:06:28,210 --> 00:06:25,680 1 to 10 electron volts where you're no 125 00:06:31,630 --> 00:06:28,220 longer ionizing 126 00:06:35,370 --> 00:06:31,640 anything and so we propose the same 127 00:06:38,230 --> 00:06:35,380 thing as what trust really organisms do 128 00:06:42,760 --> 00:06:38,240 basically having a cytochrome C analog 129 00:06:47,260 --> 00:06:42,770 which is what geo vector uses to capture 130 00:06:52,120 --> 00:06:47,270 electrons having the electrons come in 131 00:06:55,210 --> 00:06:52,130 and reducing your compounds that way we 132 00:06:58,300 --> 00:06:55,220 also had a secondary hypothesis that we 133 00:07:00,940 --> 00:06:58,310 worked on if we called in direct electro 134 00:07:03,700 --> 00:07:00,950 fiol so we gave it another name floor 135 00:07:07,420 --> 00:07:03,710 synthesis and meaning will become clear 136 00:07:10,390 --> 00:07:07,430 in just a second here so with the 137 00:07:13,210 --> 00:07:10,400 particle radiation coming in and forming 138 00:07:16,570 --> 00:07:13,220 secondary electrons we also proposed 139 00:07:19,360 --> 00:07:16,580 that the electrons might cause another 140 00:07:22,750 --> 00:07:19,370 molecule to Flores or release photons 141 00:07:24,760 --> 00:07:22,760 and then that case you would just have a 142 00:07:27,240 --> 00:07:24,770 process directly analogous to a 143 00:07:31,710 --> 00:07:27,250 traditional photosynthesis with the 144 00:07:36,250 --> 00:07:31,720 organisms using the fluorescent photons 145 00:07:38,890 --> 00:07:36,260 to power life and so this is kind of a 146 00:07:41,620 --> 00:07:38,900 diagram of what happens here so you have 147 00:07:43,719 --> 00:07:41,630 an electron or another particle that 148 00:07:46,890 --> 00:07:43,729 comes in and there's a basically a 149 00:07:50,650 --> 00:07:46,900 cascade of electrons that comes through 150 00:07:53,380 --> 00:07:50,660 and then each lower in energy than that 151 00:07:54,880 --> 00:07:53,390 first initial one you've got most going 152 00:07:58,360 --> 00:07:54,890 further down into the eyes but you have 153 00:08:00,340 --> 00:07:58,370 some escaping but we needed a way to 154 00:08:02,710 --> 00:08:00,350 quantify the number of secondary 155 00:08:06,850 --> 00:08:02,720 electrons produced so we could come up 156 00:08:10,750 --> 00:08:06,860 with a current and determine how much 157 00:08:13,300 --> 00:08:10,760 biomass we could expect to form so we 158 00:08:16,300 --> 00:08:13,310 start off with this equation here with 159 00:08:21,610 --> 00:08:16,310 this little epsilon here basically being 160 00:08:25,000 --> 00:08:21,620 how much energy is being transferred to 161 00:08:27,490 --> 00:08:25,010 the ice with each secondary electron 162 00:08:29,770 --> 00:08:27,500 that's produced and this just describes 163 00:08:32,709 --> 00:08:29,780 how much energy is being lost here as 164 00:08:36,730 --> 00:08:32,719 the electrons going through the eyes so 165 00:08:40,540 --> 00:08:36,740 what are these two values this one comes 166 00:08:45,040 --> 00:08:40,550 out to about 25 electron volts 167 00:08:48,610 --> 00:08:45,050 and then here we can estimate this value 168 00:08:51,520 --> 00:08:48,620 here by using this equation this energy 169 00:08:56,530 --> 00:08:51,530 is the energy of this initial electron 170 00:09:00,280 --> 00:08:56,540 right here and then R is just this 171 00:09:04,320 --> 00:09:00,290 equation here with this just being the 172 00:09:07,990 --> 00:09:04,330 density and this also being the initial 173 00:09:11,560 --> 00:09:08,000 electron energy here so we can recombine 174 00:09:13,990 --> 00:09:11,570 these equations to get derive a number 175 00:09:14,680 --> 00:09:14,000 of the secondary electrons coming 176 00:09:18,850 --> 00:09:14,690 through here 177 00:09:22,600 --> 00:09:18,860 so we made some estimates are using this 178 00:09:24,550 --> 00:09:22,610 and so we already have here the 179 00:09:28,120 --> 00:09:24,560 secondary electron flux how we 180 00:09:30,520 --> 00:09:28,130 calculated that so I just started off by 181 00:09:33,250 --> 00:09:30,530 looking at okay let's say we have a 182 00:09:35,970 --> 00:09:33,260 hundred particles coming in looking at 183 00:09:38,800 --> 00:09:35,980 on both a water and nitrogen Isis both 184 00:09:41,460 --> 00:09:38,810 common in the outer solar system 185 00:09:45,340 --> 00:09:41,470 Europa being an example of a water ice 186 00:09:48,430 --> 00:09:45,350 Pluto and Triton Neptune's moon being 187 00:09:50,650 --> 00:09:48,440 examples of nitrogen Isis calculating 188 00:09:54,850 --> 00:09:50,660 the penetration death using a program 189 00:09:56,920 --> 00:09:54,860 called East R which is of usually 190 00:09:58,600 --> 00:09:56,930 available unless the government's going 191 00:10:03,820 --> 00:09:58,610 down through a shutdown like it was a 192 00:10:05,920 --> 00:10:03,830 couple years ago and are using this 193 00:10:09,760 --> 00:10:05,930 secondary electron flux and this 194 00:10:12,480 --> 00:10:09,770 penetration adept or we can come up with 195 00:10:16,150 --> 00:10:12,490 the number of electrons flowing through 196 00:10:18,910 --> 00:10:16,160 and we can convert this to a current 197 00:10:22,030 --> 00:10:18,920 using the definition of what an ampere 198 00:10:23,860 --> 00:10:22,040 is so it's six point two four one times 199 00:10:28,780 --> 00:10:23,870 ten eighteenth particle charged 200 00:10:31,000 --> 00:10:28,790 particles moving per second and then we 201 00:10:35,860 --> 00:10:31,010 had we had to come up with a conversion 202 00:10:38,650 --> 00:10:35,870 factor for converting the movement of 203 00:10:42,720 --> 00:10:38,660 electrons to our grams of biomass we did 204 00:10:46,450 --> 00:10:42,730 that by basically just looking at 205 00:10:49,030 --> 00:10:46,460 photosynthesis and treating it as a 206 00:10:51,069 --> 00:10:49,040 transfer of electrons that's essentially 207 00:10:54,160 --> 00:10:51,079 what life is after all just 208 00:10:59,920 --> 00:10:54,170 transfer of electrons that we just used 209 00:11:01,949 --> 00:10:59,930 the wavelengths of light converting it 210 00:11:03,850 --> 00:11:01,959 that to how many electrons were being 211 00:11:08,769 --> 00:11:03,860 transferred within the photosynthetic 212 00:11:12,009 --> 00:11:08,779 process and coming up with the biomass 213 00:11:17,639 --> 00:11:12,019 this way and then the estimated number 214 00:11:20,859 --> 00:11:17,649 of cells is just based off how much the 215 00:11:24,359 --> 00:11:20,869 based off the weight of an e coli so 216 00:11:28,840 --> 00:11:24,369 coming up with this conversion factor 217 00:11:31,059 --> 00:11:28,850 and then this is a letter shown here 218 00:11:35,460 --> 00:11:31,069 with a graph here so just starting off 219 00:11:37,419 --> 00:11:35,470 with how many particles we had and just 220 00:11:39,910 --> 00:11:37,429 starting off with our initial or 221 00:11:43,419 --> 00:11:39,920 radiation flux and just moving forward 222 00:11:46,689 --> 00:11:43,429 we can come up with an estimated biomass 223 00:11:49,449 --> 00:11:46,699 so definitely probably overshooting here 224 00:11:52,749 --> 00:11:49,459 if the average surface of Io density on 225 00:11:59,220 --> 00:11:52,759 earth is being exceeded by Europa and 226 00:12:04,419 --> 00:11:59,230 Ganymede but and so here are we showing 227 00:12:08,860 --> 00:12:04,429 the two different types of Isis but both 228 00:12:12,910 --> 00:12:08,870 pretty comparable here so we didn't 229 00:12:17,650 --> 00:12:12,920 account for of course nutrient sources 230 00:12:19,269 --> 00:12:17,660 or anything like that but I'm going to 231 00:12:22,030 --> 00:12:19,279 move on to the relevance of the 232 00:12:25,600 --> 00:12:22,040 calculations and just a little bit and 233 00:12:27,970 --> 00:12:25,610 then same thing here estimating the 234 00:12:30,400 --> 00:12:27,980 biomass from indirect Electrify so this 235 00:12:33,309 --> 00:12:30,410 again being what we also termed as 236 00:12:36,220 --> 00:12:33,319 florist synthesis so it turns out that 237 00:12:39,369 --> 00:12:36,230 ice fluoresce is when you irradiate it 238 00:12:43,689 --> 00:12:39,379 with electrons when the wave numbers 239 00:12:47,439 --> 00:12:43,699 that comes out is or photons that comes 240 00:12:50,949 --> 00:12:47,449 out is 306 it is that found at 306 241 00:12:53,590 --> 00:12:50,959 nanometers so there was already some 242 00:12:56,340 --> 00:12:53,600 work done with the photon yield was from 243 00:13:00,380 --> 00:12:56,350 that we also converted that to its 244 00:13:04,590 --> 00:13:00,390 energy value in electron volts and 245 00:13:08,310 --> 00:13:04,600 created an electron yield based off of 246 00:13:09,600 --> 00:13:08,320 that and then did same calculations here 247 00:13:11,880 --> 00:13:09,610 when just based off the amount of 248 00:13:15,030 --> 00:13:11,890 photons of being our producer just 249 00:13:22,550 --> 00:13:15,040 assuming that one of these photons would 250 00:13:25,170 --> 00:13:22,560 be equivalent to when terrestrial photon 251 00:13:27,650 --> 00:13:25,180 that's used sir that's used in 252 00:13:30,810 --> 00:13:27,660 terrestrial photosynthesis but also used 253 00:13:34,920 --> 00:13:30,820 one based off the energy flux from those 254 00:13:37,680 --> 00:13:34,930 photons are higher in energy being 255 00:13:39,210 --> 00:13:37,690 supplied with a little more but in terms 256 00:13:42,449 --> 00:13:39,220 of an order of magnitude doesn't really 257 00:13:45,690 --> 00:13:42,459 make much of a difference in terms of 258 00:13:49,160 --> 00:13:45,700 how much IU form so it shows the same 259 00:13:53,759 --> 00:13:49,170 thing here with the radiation flux I 260 00:13:57,030 --> 00:13:53,769 just going out here showing how much you 261 00:14:00,660 --> 00:13:57,040 would expect in terms of biomass and 262 00:14:03,180 --> 00:14:00,670 again lower than the average surface 263 00:14:05,939 --> 00:14:03,190 biomass density on earth but still 264 00:14:09,060 --> 00:14:05,949 fairly high here again not accounting 265 00:14:09,769 --> 00:14:09,070 for on your nutrients or anything like 266 00:14:12,210 --> 00:14:09,779 that 267 00:14:17,340 --> 00:14:12,220 so these estimates are definitely high 268 00:14:19,650 --> 00:14:17,350 so how reliable are these so one of the 269 00:14:21,720 --> 00:14:19,660 things that we first things that we did 270 00:14:25,310 --> 00:14:21,730 in Spain was we were actually looking at 271 00:14:29,310 --> 00:14:25,320 beta decay first and with beta decay 272 00:14:32,790 --> 00:14:29,320 when we applied these same calculations 273 00:14:35,519 --> 00:14:32,800 here didn't really get substantial 274 00:14:38,730 --> 00:14:35,529 amounts of biomass that were able to be 275 00:14:40,889 --> 00:14:38,740 supported until we looked at these guys 276 00:14:45,090 --> 00:14:40,899 here and these are waste products from 277 00:14:48,269 --> 00:14:45,100 on nuclear reactors and so we also see 278 00:14:50,790 --> 00:14:48,279 high biomass estimates based off those 279 00:14:56,850 --> 00:14:50,800 and it's interesting because just 280 00:14:59,130 --> 00:14:56,860 recently and they found a fun guy in a 281 00:15:05,100 --> 00:14:59,140 Chernobyl Ukraine that are able to 282 00:15:07,800 --> 00:15:05,110 utilize a radiation coming off from 283 00:15:10,290 --> 00:15:07,810 there the exact mechanism is still 284 00:15:12,600 --> 00:15:10,300 unknown but even though these 285 00:15:16,220 --> 00:15:12,610 calculations you know 286 00:15:19,559 --> 00:15:16,230 aren't giving probably aren't giving us 287 00:15:22,799 --> 00:15:19,569 the exact biomass readings here they 288 00:15:25,259 --> 00:15:22,809 might be indicative of they might still 289 00:15:27,840 --> 00:15:25,269 be helpful in determining and telling 290 00:15:32,759 --> 00:15:27,850 you you know like a pointer like hey 291 00:15:39,119 --> 00:15:32,769 life might exist here or not and so how 292 00:15:41,160 --> 00:15:39,129 would life survive on subsurface moon so 293 00:15:43,579 --> 00:15:41,170 I already talked about the particle 294 00:15:47,179 --> 00:15:43,589 radiation here forming secondary 295 00:15:51,900 --> 00:15:47,189 electrons and that energy just being 296 00:15:55,679 --> 00:15:51,910 dissipated here into a smaller or more 297 00:15:58,619 --> 00:15:55,689 electrons but with smaller packets of 298 00:16:02,879 --> 00:15:58,629 energy that wouldn't be involved in 299 00:16:06,389 --> 00:16:02,889 ionizing chemistry okay sorry 300 00:16:09,119 --> 00:16:06,399 so also forming spores here on the 301 00:16:12,869 --> 00:16:09,129 surface so if ice gets sublimated and 302 00:16:15,420 --> 00:16:12,879 you can't form secondary electrons you 303 00:16:22,499 --> 00:16:15,430 can go into a spore mode ice nucleation 304 00:16:24,150 --> 00:16:22,509 proteins that basically help form ice 305 00:16:27,739 --> 00:16:24,160 outside the cell instead of the inside 306 00:16:32,519 --> 00:16:27,749 and then antifreeze proteins that 307 00:16:35,009 --> 00:16:32,529 basically don't keep your organism from 308 00:16:37,949 --> 00:16:35,019 freezing and in terms of testing the 309 00:16:39,809 --> 00:16:37,959 hypothesis the easiest way right now 310 00:16:43,799 --> 00:16:39,819 would be to use one of the terrestrial 311 00:16:46,259 --> 00:16:43,809 electrophilic organisms and then using 312 00:16:48,720 --> 00:16:46,269 these secondary electrons to power them 313 00:16:50,910 --> 00:16:48,730 and so basically just went over the kind 314 00:16:53,699 --> 00:16:50,920 of back of the envelope estimates that 315 00:16:55,860 --> 00:16:53,709 we had for direct and indirect electro 316 00:17:00,379 --> 00:16:55,870 fee and now we're just revising our 317 00:17:03,019 --> 00:17:00,389 biomass estimates and putting in some 318 00:17:05,750 --> 00:17:03,029 electrochemistry thermodynamics before 319 00:17:12,210 --> 00:17:05,760 finishing everything off and just some 320 00:17:21,159 --> 00:17:14,350 it's time for one quick question before 321 00:17:23,289 --> 00:17:21,169 we run oh you're closer sorry hi I'm 322 00:17:25,990 --> 00:17:23,299 great talk so what I was curious was 323 00:17:28,659 --> 00:17:26,000 what I guess ratio or metric did you use 324 00:17:30,580 --> 00:17:28,669 to determine the translation from the 325 00:17:36,400 --> 00:17:30,590 electron volts to the amount of biomass 326 00:17:38,440 --> 00:17:36,410 produced okay so that was okay so the 327 00:17:41,980 --> 00:17:38,450 electron volts in terms of the initial 328 00:17:46,620 --> 00:17:41,990 particle energy I'll back up here so 329 00:17:50,169 --> 00:17:46,630 it's not the energy of the electron here 330 00:17:54,580 --> 00:17:50,179 that determines how much energy the 331 00:17:58,570 --> 00:17:54,590 organism unnecessarily gets the electron 332 00:18:00,909 --> 00:17:58,580 volts here are important because they 333 00:18:04,690 --> 00:18:00,919 decide how many secondary electrons are 334 00:18:06,669 --> 00:18:04,700 produced and then that in itself 335 00:18:10,450 --> 00:18:06,679 produces a current here and then we use 336 00:18:12,970 --> 00:18:10,460 that current then to determine how much 337 00:18:14,590 --> 00:18:12,980 biomass and was that from the previous 338 00:18:16,840 --> 00:18:14,600 values of electrophilic organizing 339 00:18:18,909 --> 00:18:16,850 organisms on earth I know so with 340 00:18:21,760 --> 00:18:18,919 electrophilic organisms it's a little 341 00:18:26,950 --> 00:18:21,770 bit more straightforward because you're 342 00:18:32,020 --> 00:18:26,960 using cells so you can come up with the 343 00:18:34,840 --> 00:18:32,030 exact energy here more directly but with 344 00:18:37,270 --> 00:18:34,850 the plasma here it's a little bit 345 00:18:42,240 --> 00:18:37,280 different so we decided to use the